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Glycated Albumin

Analyte: Glycated Albumin

Specimen Type: Serum, plasma (EDTA, heparin, or citric acid)

Optimum Volume: 0.5 mL

Stability:

2-8°C -20°C -70°C
1 week 1 month 3 years

Reporting units: g/dL

Method: Enzymic

Biological or Clinical Significance:

Glycation is the result of a sugar molecule, such as fructose or glucose, bonding to a protein or lipid molecule without the controlling action of an enzyme. It is a haphazard process that impairs the functioning of biomolecules. The high levels of glucose present in diabetes mellitus results in increased glycation of all proteins, including, albumin. Measurement of the amount of the glycation reaction resulting from the combination of glucose with free amino groups in proteins present in the blood is used to monitor the level of glucose that has generally been present in body fluids over a preceding period. Thus serum glycated albumin assay can be used to determine the current level of glycation of albumin, the most abundant plasma protein. Hence, the glycated albumin assay may be used as a marker of glycemic control in diabetes and reflects glycemic control over the previous 2 to 4 weeks.

Principle of Test Method:

The glycated albumin assay is an automated enzymic method.

References:

1. Armbuster DA. Fructosamine: Structure, analysis, and clinical usefulness. Clin Chem 1971; 33:2153-2163.
2. Takahashi S, Uchino H, Shimuzu T, Kanazawa A, Tamura Y, Sakai K, Watada K, Hirose T, Kawamori R, Tanaka Y. Comparison of glycated albumin (GA) and glycated hemoglobin for evaluation of short-term changes in glycemic control. Endocrine J 2007; 54:139-144.
3. Inaba M, Okuno S, Kumeda Y, Yamada S, Imanishi Y, Tabata T, Okamura M, Okada S, Yamakawa T, Ishimura E, Nishizawa Y. Glycated albumin is a better glycemic indicator than glycated hemoglobin values in hemodialysis patients with diabetes: Effect of anemia and erythropoietin injection. J Am Soc Nephrol 2007; 18:896-903.
4. Wu C, Carlson TH, Roe ND, Leary ET. Evaluation of a direct enzymatic glycated albumin assay on the Roche/Hitachi Modular P chemistry analyzer. Clin Chem. 2007; 53(Suppl): A189-A190.
5. Kouzuma T, Usami T, Uamakoshi M, Takahashi M, Imamura S. An enzymatic method for the measurement of glycated albumin in biological samples. Clin Chim Acta 2002; 324:61-71.
6. Kouzuma T, Uemastu Y, Usami T, Imamura S. Study of glycated amino acid elimination reaction for an improved enzymatic glycated albumin measurement method. Clin Chim Acta 2004; 346:135-143.
7. Muramoto Y, Matsushita M, Irino T. Reduction of reaction differences between human mercaptalbumin and human nonmercaptoalbumin measured by the bromcresol purple method. Clin Chim Acta. 1999; 289:69-78.
8. Shima K, Ito N, Abe F, Hirota M, Yano M, Yamamoto Y, Uchida T, Noguchi K. High-performance liquid chromatographic assay of serum glycated albumin. Diabetologia. 1988; 31:
9. Miyamoto N, Shirakawa N, Kuroda Y, Abe F, Shima K. Serum levels of glycated albumin in non-diabetic and insulin-dependent diabetic children. Acta Paediatr Jpn; 32:249 – 256.

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